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Short Interfering RNA–Mediated Gene Silencing of Vascular Endothelial Growth Factor
Effects on Cellular Proliferation in Colon Cancer Cells
Abby L. Mulkeen, MD;
Teresa Silva, BS;
Peter S. Yoo, MD;
John C. Schmitz, PhD;
Edward Uchio, MD;
Edward Chu, MD;
Charles Cha, MD
Arch Surg. 2006;141:367-374.
Hypothesis By using short interfering RNA (siRNA) to inhibit the in vitro expression of vascular endothelial growth factor (VEGF) A, we hope to further investigate the presence of an autocrine loop in colon cancer cells. We hypothesize that VEGF inhibition will result in decreased cellular proliferation.
Design Human colon cancer cells were evaluated for the expression of VEGF and VEGF receptor 2 (VEGFR-2). In vitro assessments were then made of the ability of anti-VEGF siRNA to knock down expression of VEGF and the subsequent effect this decreased expression had on colon cancer cell proliferation.
Setting Surgical oncology research laboratory.
Interventions Human colon cancer cells from the RKO cell line were transfected with siRNA targeting the coding region of VEGF.
Main Outcome Measures Enzyme-linked immunosorbent assay, Northern blot analysis, and real-time quantitative polymerase chain reaction were performed to establish the ability of siRNA to decrease VEGF production. Proliferation assays were run on transfected and wild-type cells to establish concomitant decrease in VEGF expression and cellular proliferation.
Results The RKO colon cancer cells expressed both VEGF and VEGFR-2. Those cells transfected with siRNA targeting VEGF showed a 94% knockdown in VEGF expression and a 67% decrease in cellular proliferation.
Conclusion Colon cancer cells expressing VEGF and VEGFR-2 may possess an autocrine growth pathway that can be effectively targeted using RNA interference as an antiangiogenic therapy.
Author Affiliations: Department of Surgery (Drs Mulkeen, Yoo, and Cha and Ms Silva), Department of Medicine and Pharmacology, Yale Cancer Center (Drs Schmitz and Chu), and Department of Urology (Dr Uchio), Yale University School of Medicine, New Haven, Conn; and Department of Surgery (Drs Mulkeen, Yoo, and Cha and Ms Silva), Veterans Affairs Connecticut Cancer Center (Drs Schmitz and Chu), Veterans Affairs Connecticut Healthcare System, West Haven.
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