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WILLIAM C. LINEAWEAVER, MD
San Francisco

Arch Surg. 1988;123(10):1286.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

To the Editor.—In their article in the April 1988 issue of the ARCHIVES, Kozol et al¹ cite an earlier study by me and my colleagues.² Their discussion, however, misinterprets two points in our article and makes the differences in our findings appear greater than they are.

First, Kozol et al state that our study "was based on the trypan blue exclusion test for cell viability." In fact, we used cell survival in culture as our viability assay^2,3 and found trypan blue staining to be a relatively insensitive indicator of cell damage, as Kozol et al found when they compared trypan blue staining with electron microscopy. The second error occurs when Kozol et al state that we "suggest that at lower concentrations (5x10^–2%) of Dakin's solution, cultured fibroblasts are not killed." In our study, we found intact fibroblast viability at 0.5x10^–2%, or 5x10^–3%, a . . . [Full Text PDF of this Article]

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