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ISIDORE COHN, Jr., M.D.; GEORGE J. WERNETTE, M.D.

AMA Arch Surg. 1957;75(6):1003-1005.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Difficulty in obtaining large quantities of sterile bile has led to the development of several techniques in various animal species for prolonged collection of bile. The continuing sterility of these methods is open to serious question.

In the rat, which has no gallbladder, bile has been collected by cannulating the common duct and allowing bile to drain into specially constructed glass bulbs which were placed either in the peritoneal cavity^1,2 or on the abdominal wall.³ Bile was withdrawn at specified intervals from the glass bulbs through rubber tubes. Another technique used a long plastic tube extending from the common duct through a subcutaneous tunnel to the dorsal surface of the rat's foot to permit continuous collection of bile while the animal was moving about.⁴

In the rabbit the common duct has been divided between ligatures, and a flexible tube inserted into the gallbladder and brought to the . . . [Full Text PDF of this Article]

Author Affiliations
New Orleans

From the Department of Surgery, Louisiana State University School of Medicine.

Footnotes
Submitted for publication May 20, 1957.

Supported in part by a grant from the National Microbiological Institute of the National Institutes of Health, U. S. Public Health Service (No. E-524-C2).

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