This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on Web of Science (32)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

ARMAND M. KAROW, JR., BA; WATTS R. WEBB, MD; JOHN E. STAPP

AMA Arch Surg. 1965;91(4):572-574.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

PRESERVATION of mammalian organs by hypothermia requires methods of cooling and warming that will maintain uniform temperature distribution throughout the tissues, yet permit changes in temperature at a sufficiently rapid rate to equalize physical stress and minimize variations in biochemical reaction rates.³ The freezing of cells has been most successful when performed relatively slowly (in the range of 1 to 10 C per minute) in the temperature range from 0 to 20 C with rapid freezing thereafter. This present work has sought the limits of freezing of organs with best available techniques and protective agents.

Methods

Albino rats averaging 200 gm received 25 mg pentobarbital (Nembutal) and 5 mg sodium heparin intraperitoneally. The aorta was severed and an aortic cannula was lodged just above the coronary arteries. The heart and lungs were removed in toto and placed on a modified Langendorff perfusion column (Fig 1). After perfusion commenced the . . . [Full Text PDF of this Article]

Author Affiliations
DALLAS

From the Department of Surgery, Southwestern Medical School, University of Texas.

Footnotes
Submitted for publication March 13, 1965.

Reprint requests to 5323 Harry Hines Blvd, Dallas, Tex 75235 (Dr. Webb).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?